Amino Acid Analysis of given Sample (without total hydrolysis)

set-up

Amino acid analysis of given protein/peptide probe without acid hydrolysis. For analysis we do need 1.0 µg to 5.0 µg (20 pmols to 100 pmols) protein, based on an average molecular weight of 25,000 Da. For peptides, the requirement is much smaller - 0.2 µg to 1.0 µg (200 pmols to 1,000 pmols).

The amino acid analysis service encloses the total hydrolyis of a given sample, the chemical derivatisation of the free amino acids (either produced by the acid hydrolysis or of already free amino acids), the separation of the derivatised amino acids by reversed phase HPLC and subsequent interpretation of the result. Pure samples are required. The presence of salts, buffers, or detergents are deleterious. Amines (primary or secondary) will react with the carbamate, adversely affecting results. While salts, especially strong buffer substances can alter the pH of the sample causing the hydrolysis/derivitisation to be incomplete or simply fail. Additionally, significant levels of glycerol or carbohydrates are problematic - the glycerol is nonvolatile and attracts moisture (acid) and carbohydrates char, decomposing to ash taking the sample with them.

Productnumber: P2145.0002

Productdetails:

  Amino Acid Analysis of given Sample (without total hydrolysis) set-up

Deutsche Produktseite:
Aminosäureanalyse (ohne Totalhydrolyse) set-up  Services

Matchcode: amino acids AAA aminoacid amino acid analysis Aminosäureanalyse