Hygromycin B Powder

1 g

O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-beta-D-talopyranosyl-(1-5)-2-deoxy-N3-methyl-D-streptamine. Aminoglycoside antibiotic.

Aminoglycoside antibiotic that inhibits growth of procaryotic micro-organisms (bacteria), eukaryotic micro-organisms (yeast) and mammalian cells. Inhibits protein synthesis at translocation step: causes misreading of mRNA. A gene from E.coli encoding resistance to hygromycin B can be isolated and cloned by recombinant DNA technology. It is useful for the identification or selection of recombinant clones in various cell types. The working concentration for mammalian cell selection is normally between 50 µg/mL and 1 mg/mL, plant cells: 20 µg/mL-200 µg/mL, bacteria: 20 µg/mL-200 µg/mL and Fungi: 200 µg/mL - 1 mg/mL. The appropriate concentration should be tested experimentally.

Documentation:

  Hygromycin B Powder 1 g

Productnumber: M3125.0001

Manualcertificate:

  Hygromycin B Powder 1 g

Technical Data: "min. 90%; specific activity: 1x10(6) U/g; substance is soluble in water; C20H37N3O13; MW = 527.53 g/mol; CAS [31282-04-9]; toxic/harmful; may be cancerogenic or teratogenic; working solutions (< 2mg/mL) are stable for about 1 month at +4°C, it is recommended to use end concentrations between 100 µg/mL and 800 µg/mL."

Shipping/Storing-Information: shipped and stored at RT

Deutsche Produktseite:
Hygromycin B Pulver 1 g  Cell Biology

Matchcode: aminoglycoside misreading of mRNA hygromycin