Tiamutin, 1mg/mL 50 mL

Tiamuline for treatment of mycoplasm contaminated cell culture medium.

Tiamutin binds to ribosomal 50S subunit and interferes with the peptidyl transferase activity. Tiamutin binds in the 'Peptidyl-Transferase-Center' (PTC) at the entrance of the ribosomal tunnel. Binding is facilitated by the 3-fold structure of the mutilin-core of the Tiamutin molecule. The bond Tiamutin molecule reaches from the Mutilin core to the other side of the catalytic centre, interfering with the binding site for P-tRNA. Thus Tiamutin inhibits directly the generation of the peptide chain (Drews et al., 1975; Egger and Reinshagen, 1976a; Hodgin and Hogenauer, 1974; Hogenauer, 1975). On the other hand, protein synthesis cycle prevents binding of Tiamutin (Dornhelm and Hogenauer, 1978), as the A- or P-binding domain for tRNA, can not be occupied by Tiamutin anymore. IUPAC-name: (4R,5S,6S,8R,9aR,10R)-5-Hydroxy-4,6,9,10-tetramethyl-1-oxo-6-vinyldecahydro-3a,9-propanocyclopenta[8]annulen-8-yl {[2-(diethylamino)ethyl]sulfanyl}acetate.