CHAPS for 2D-gel electrophoresis

50 g

CHAPS - (3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate)

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150 Da) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).

Productnumber: M6201.0050

Technical Data: "Purity: >99.0% (HPLC); MW 614.89; White solid crystals; CMC 4.2 - 6.3 mmol/L; Aggregation No.: 10; Absorbance (1 cm / 5%, H2O) 290 nm < 0.5; 400 nm 0.02; Pb < 0.005%; Na < 0.005%; N 4.5%; H2O: max. 3%; conductivity: max. 10uS/cm (25°C, 1% solution); C32H58N2O7S; CAS: [75621-03-3]."

Shipping/Storing-Information: shipped at RT, store at +2°C to +8°C

Deutsche Produktseite:
CHAPS für die 2D-Gelelektrophorese 50 g  Biochemicals

Matchcode: CHAPS (3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate) 2D IEF