Collagenase Typ I 1 g

Collagenase from Clostridium histolyticum lyophilised (125 to 250 Mandl-Units). Histolyticum clostridiopeptidase (EC 3.4.24.3).

Natural balance of enzyme activity. Recommended for cell preparation from epithelial, liver, lung tissue, tissue of the suprarenal gland and adipose tissue. Genaxxon Collagenase from Clostridium histolyticum also contain a number of other enzymatic activities, including clostripain, which cleaves peptides preferentially at the carboxyl side of arginine residues, a tryptic activity, which acts preferentially at the carboxyl side of arginine and lysine residues, and neutral non-specific proteases. Specific activity of 125 to 250 Mandl-Units per mg dry substance. Collagenase requires calcium ions both for full catalytic activity and binding to the collagen molecule. Collagenase is soluble in water and diluted salt solutions. The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl and 2,3-dimercaptopropanol. It is also inhibited by TRIS buffer above pH7.5. Collagenase is not inhibited by diisopropylphosphorofluoridate. Lyphilised Collagenase can be stored at +4°C and remain stable without loss of activity for at least three years. Enzymes should be protected from moisture. Dissolved enzymes can be aliquoted and stored at -20°C for one year. Repeated freezing and thawing should be avoided. Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).