Cloning
Services
cDNA cloning - Cloning of PCR product - Cloning of synthetic DNA - in vitro Mutagenesis - Library construction - Plasmid preparation (up to 10 mg) - Plasmid preparation (up to 10 µg) - Plasmid preparation (up to 100 µg) - Plasmid preparation (up to 500
cDNA cloning set-up
3095,41 € (EUR) - Synthesis and cloning of cDNA up to 1 kbp. For cloning of fragments > 1 kbp we bill 0.76 Euro per bp. - P2008.0000
Cloning of PCR product clone
1548,32 € (EUR) - Primer design and synthesis. PCR with high fidelity polymerase and cloning into vector. Verification by sequencing, documentation. - P2002.0002
Cloning of synthetic DNA set-up
848,48 € (EUR) - Price for small synthetic DNA-constructs (up to 100 bases). - P2144.0000
in vitro Mutagenesis set-up
1216,05 € (EUR) - Site directed mutagenesis (plasmid based, plasmid < 5000 bp). - P2012.0000
Library construction set-up
Price on request - Construction of cDNA library according to customer demand. - P2001.0000
Plasmid preparation (up to 10 mg) per plasmid
1102,41 € (EUR) - Plasmid preparation from up to 10 mg DNA (with high copy plasmid). - P2009.1010
Plasmid preparation (up to 10 µg) per plasmid
15,61 € (EUR) - Plasmid preparation from up to 10 µg DNA (with high copy plasmid). - P2009.0010
Plasmid preparation (up to 100 µg) per plasmid
93,83 € (EUR) - Plasmid preparation from up to 100 µg DNA (with high copy plasmid). - P2009.0100
Plasmid preparation (up to 500 µg) per plasmid
341,56 € (EUR) - Plasmid preparation from up to 500 µg DNA (with high copy plasmid). - P2009.0500
Subcloning (blunt end, < 2000 bp) clone
713,16 € (EUR) - Subcloning of blunt-end DNA from one vector into an other using common restriction enzymes. Confirmation by restriction analysis. - P2005.0002
Subcloning (difficult enzymes or fragments > 2000 bp) clone
Price on request - Subcloning of DNA from one vector into an other using difficult restriction enzymes or fragment sizes > 2000 bp. Confirmation by restriction analysis. - P2005.0003
Subcloning (sticky end, < 2000 bp) clone
626,45 € (EUR) - Subcloning of sticky DNA from one vector into an other using common restriction enzymes. Confirmation by restriction analysis. - P2005.0001
Synthetic genes bp
6,96 € (EUR) - Synthesis of dsDNA and cloning into PCR cloning vector, verification by sequencing. - P2007.0000
Transformation of plasmid-DNA per plasmid
40,97 € (EUR) - Transformation of Plasmid-DNA into organism. - P2010.0000
Verification sequencing (< 300 bp) clone
63,73 € (EUR) - sequencing of cloned construct - P2003.0001
Verification sequencing (> 300 bp) per base
0,24 € (EUR) - sequencing of cloned construct - P2003.0002
