Cloning

Services

Cloning Section

cDNA cloning set-up
2499,00 € (EUR) - Synthesis and cloning of cDNA up to 1 kbp. For cloning of fragments > 1 kbp we bill 0.76 Euro per bp. - P2008.0000

Cloning of eukaryotic expression vector set-up
992,25 € (EUR) - PCR amplification of requested DNA-fragment. Cloning into expression- or reporter vector. Production and purification of vector. Sequencing and restriction digest for quality check. - P2041.0001

Cloning of PCR product clone
1250,00 € (EUR) - Primer design and synthesis. PCR with high fidelity polymerase and cloning into vector. Verification by sequencing, documentation. - P2002.0002

Cloning of synthetic DNA set-up
685,00 € (EUR) - Price for small synthetic DNA-constructs (up to 100 bases). - P2144.0000

in vitro Mutagenesis set-up
981,75 € (EUR) - Site directed mutagenesis (plasmid based, plasmid < 5000 bp). - P2012.0000

Library construction set-up
Price on request - Construction of cDNA library according to customer demand. - P2001.0000

Plasmid preparation (up to 10 mg) per plasmid
890,00 € (EUR) - Plasmid preparation from up to 10 mg DNA (with high copy plasmid). - P2009.1010

Plasmid preparation (up to 10 µg) per plasmid
12,60 € (EUR) - Plasmid preparation from up to 10 µg DNA (with high copy plasmid). - P2009.0010

Plasmid preparation (up to 100 µg) per plasmid
75,75 € (EUR) - Plasmid preparation from up to 100 µg DNA (with high copy plasmid). - P2009.0100

Plasmid preparation (up to 500 µg) per plasmid
275,75 € (EUR) - Plasmid preparation from up to 500 µg DNA (with high copy plasmid). - P2009.0500

Subcloning (blunt end, < 2000 bp) clone
575,75 € (EUR) - Subcloning of blunt-end DNA from one vector into an other using common restriction enzymes. Confirmation by restriction analysis. - P2005.0002

Subcloning (difficult enzymes or fragments > 2000 bp) clone
Price on request - Subcloning of DNA from one vector into an other using difficult restriction enzymes or fragment sizes > 2000 bp. Confirmation by restriction analysis. - P2005.0003

Subcloning (sticky end, < 2000 bp) clone
505,75 € (EUR) - Subcloning of sticky DNA from one vector into an other using common restriction enzymes. Confirmation by restriction analysis. - P2005.0001

Subcloning of customer construct into baculovirus transfer vector. clone
1888,95 € (EUR) - Removing of gene of interest from original vector, or amplification of PCR fragment of the gene of interest. - P2016.0000

Synthetic genes bp
5,62 € (EUR) - Synthesis of dsDNA and cloning into PCR cloning vector, verification by sequencing. - P2007.0000

Transformation of plasmid-DNA per plasmid
33,08 € (EUR) - Transformation of Plasmid-DNA into organism. - P2010.0000

Verification sequencing (< 300 bp) clone
51,45 € (EUR) - sequencing of cloned construct - P2003.0001

Verification sequencing (> 300 bp) per base
0,19 € (EUR) - sequencing of cloned construct - P2003.0002