rec. Human Fibroblast Growth Factor-basic (rHuFGF-b) 1 mg

Lyophilized from a concentrated (1mg/mL) sterile solution containing 5mM Tris pH=7.5 and 150 mM NaCl. Synonyms: Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Recombinant Human FGF-b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17,353 Dalton. The ED50, calculated by the dose-dependant proliferation of BAF3 cells (measured by 3H-thymidine uptake) is 0.3ng/mL (Limit of acceptance: 0.5ng/mL), corresponding to a specific activity of 3.4 x 10(6) Units/mg. Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.