rec. Human Interleukin-10 (rHuIL-10) 1 mg

Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. Recombinant Human IL-10 produced in CHO is a non covalent dimer of 2 polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Interleukin-10 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be < 0.5ng/mL, corresponding to a Specific Activity of 3x10E6 IU/mg.