rec. Human Interleukin-15 (rHuIL15) 2 µg

Lyophilized from a concentrated (1mg/mL) solution with 5mM Tris pH 8.0. Synonyms: IL-15, MGC9721.

Recombinant Human IL-15 produced in E.Coli is a single, non-glycosylated, polypeptide of 114 amino acids and a molecular mass of 13,007 Dalton. The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be <0.45ng/mL, corresponding to a Specific Activity of 3 x 10(6) IU/mg. The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.