Pfunds proof reading polymerase

250 units

A thermostable high fidelity enzyme possessing 5'-3' DNA polymerase and 3'-5' proof reading exonuclease activities.

The Genaxxon BioScience Pfunds-Polymerase is a thermostable enzyme possessing 5'-3' DNA polymerase and 3'-5' proof reading exonuclease activities. It is isolated from the hyperthermophilic marine archae Pyrococcus furiosis (Pfu). The enzyme provides extremely high fidelity. Whereas the enzyme is not able to amplify long fragments as efficiently as Taq-Polymerase because of its very high exonuclease activity, a mixture of Taq-Polymerase and Pfunds-Polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220).

Documentation:

  Pfunds proof reading polymerase 250 units

Productnumber: M3004.0250

Productdetails:

  Pfunds proof reading polymerase 250 units

Manual/Protocol:

  Pfunds proof reading polymerase 250 units

Application: Proof-reading DNA polymerase for high fidelity PCR.

Source: Pyrococcus furiosis

Unit-definition: "One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate."

Technical Data: 2.5 U/µL

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
Pfunds Proof-reading Polymerase 250 units  PCR-related

Matchcode: PCR pfu polymerase thermostable high fidelity proofreading proof-reading amplification