Pwo Polymerase

2 x 250 units

A highly processive 5' - 3' DNA Polymerase with additional 3' - 5' exonuclease activity (proof-reading). 10-fold higher fidelity compared to Taq-Polymerase.

Genaxxon BioScience Pwo DNA Polymerase is a highly processive 5' - 3' DNA Polymerase with additional 3' - 5' exonuclease activity (proof-reading). The Genaxxon BioScience Pwo DNA Polymerase is the recombinant form, originally isolated form the themophilic archaebacterium Pyrococcus woesei. It has been cloned and expressed in E.coli. The enzyme has no detectable 5' - 3' exonuclease activity. It exhibits increased thermal stability as well as a tenfold increase in fidelity of DNA synthesis compared to Taq DNA Polymerase. Pwo generates blunt-ended PCR products.

Documentation:

  Pwo Polymerase 2 x 250 units

Productnumber: M3002.0500

Productdetails:

  Pwo Polymerase 2 x 250 units

Manual/Protocol:

  Pwo Polymerase 2 x 250 units

Application: Proof-reading DNA polymerase for high fidelity PCR.

Source: recombinant

Unit-definition: "One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate."

Technical Data: 2.5 U/µL

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
Pwo Polymerase 2 x 250 units  PCR-related

Matchcode: PCR Pwo pwo polymerase thermostable high fidelity proofreading proof-reading amplification enzyme