ReproFast Polymerase

250 units

ReproFast is a special DNA polymerase, that exhibits proofreading activities, but also the high processivity of Taq DNA Polymerase. ReproFast is suited for PCR of DNA-fragments up to > 7 kb with high accuracy.

The Genaxxon BioScience ReproFast DNA-Polymerase is a thermostable enzyme possessing 5‘-3‘ DNA polymerase and 3‘-5‘ proof reading exonuclease activities (generates no A-overhangs/suitable for blund-end cloning). ReproFast high-fidelity DNA polymerase provides high overall performance and more robust amplification of longer targets due to Genaxxons polymerase-enhancing factor. The ReproFast polymerase improves the yield of DNA amplicons compared to that from standard Pfu- or Pwo DNA polymerase. It enhances overall PCR performance, including shorter extension times, higher yield and greater target length capability. The main application is for amplification of long target sequences, including genomic DNA giving high accuracy for cloning experiments. With ReproFast it will be possible to amplify fragments up to 5 kb from human genomic DNA and up to 7 kb from lambda DNA (Barnes (1994) Proc. Natl. Acad. Sci. USA 91. 2216-2220) with the accuracy of Pfu- or Pwo polymerases.

Documentation:

  ReproFast Polymerase 250 units

Productnumber: M3003.0250

Productdetails:

  ReproFast Polymerase 250 units

Manual/Protocol:

  ReproFast Polymerase 250 units

Application: Polymerase mixture to be used for long fragments in PCR

Source: recombinant

Unit-definition: "One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate."

Technical Data: 5 U/µL

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
ReproFast Polymerase 250 units  PCR-related

Matchcode: PCR Taq Pwo pwo polymerase blent thermostable high fidelity and processivity activities proofreading proof-reading amplification enzyme mix