Tth Polymerase

500 units

Tth DNA Polymerase is the recombinant form of the enyzme, a highly processive 5' - 3' DNA Polymerase lacking 3' - 5' exonuclease activity.

Genaxxon BioScience Tth DNA Polymerase is the recombinant form of the enzyme obtained from the thermophilic eubacterium Thermus termophilus HB-8. It is a highly processive 5' - 3' DNA Polymerase lacking 3' - 5' exonuclease activity. Tth DNA Polymerase catalyses the polymersisation of nucleotides into duplex DNA in the 5' - 3' direction in the presence of MgCl2 together with a very efficient intrinsic reverse transcriptase (RT) activity in the presence of Mn2+ ions. This RT activity is not associated with RNase H activity. The ability of Tth DNA Polymerase to catalyse the polymerisation of DNA, using a RNA template at high temperature, minimises the problems encountered with strong secondary structures in RNA, since they are unstable at higher temperatures. Tth DNA Polymerase is resistant to prolonged incubation at 95°C.

Documentation:

  Tth Polymerase 500 units

Productnumber: M3005.0500

Productdetails:

  Tth Polymerase 500 units

Manual/Protocol:

  Tth Polymerase 500 units

Source: recombinant

Unit-definition: "One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate."

Technical Data: 5 U/µL

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
Tth Polymerase 500 units  PCR-related

Matchcode: PCR Tth Taq taq polymerase thermostable thermophilus aquaticus high processivity RT PCR reverse transcriptase activity amplification enzyme