MS-Silver Staining Kit

1 Kit

The MS-Silver staining kit is a mass spectrometry compatible silver staining kit. The staining is very sensitive, results in low staining background and is highly compatible with subsequent protein identification by nanoLC-ESI-MSMS and MALDI-TOFTOF-MS.

The MS-Silverkit is a mass spectrometry compatible silver staining kit which was specially developed and optimized for high sensitive staining of protein spots and bands from 2DE and SDS-PAGE for subsequent protein identification analysis by mass spectrometry. MS-Silver stained proteins can be detected down to about 1 ng/mm2. The staining background is very low resulting in a very good signal to noise ratio. Thus MS-Silver is an appropriate alternative to silver staining methods with glutaraldehyde but without the disadvantages caused by this crosslinker. Due to MS-Silver’s high compatibility to subsequent MS analysis of protein spots it is not longer necessary to prepare an additional micro-preparative Coomassie Blue stained 2DE gel for protein identification which contain a multiple amount of sample used for silver staining. Weak protein spots only visible by MS-Silver staining are accessible to protein identification because MS-Silver is about 20-100 times more sensitive than Coomassie Blue staining methods without the disadvantages of common (even MS compatible) silver staining procedures. MS-Silver’s application is easy and results in cost reduction and saving of time together with significantly improved sensitivity in protein identification from 2DE gels and SDS-PAGE.

Productnumber: M3030.0001

Productdetails:

  MS-Silver Staining Kit 1 Kit

Application: for staining of proteins in 2D or PAGE gels

Shipping/Storing-Information: stored and shipped at RT

Deutsche Produktseite:
MS-Silver Färbekit 1 Kit  Chemicals

Matchcode: silver stain silberfärbung