Ponceau S Solution

500 mL

1% solution in 5% acetic acid.

Ponceau S is used for reversible staining proteins blotted on nitrocellulose membran and while running a TAU electrophoresis. The reversibility of the staining process is ideally suited for subsequent immunoblots (western blot) or other colour reactions as Ponceau S can be completely removed. Ponceau S is not stable for long time, thus proteins and protein markers should be marked with a pencil. For staining the membrane is incubated in a 0.1% - 0.5% Ponceau S solution in 3% trichloracetic acid for 5 - 10 minutes. Destaining is done by incubating the stained membrane in 5% acetic acid for 15 minutes.

Documentation:

  Ponceau S Solution 500 mL

Productnumber: M3350.0500

Application: for reversible staining of blotted membranes

Technical Data: density (d 20°/4°): 1.01; n 20°/D: 1.338; 5.00 g/L acedi acid; 1.0 g/L ponceau S.

Shipping/Storing-Information: shipped and stored at RT

Deutsche Produktseite:
Ponceau S Lösung 500 mL  Chemicals

Matchcode: dye stains electrophoresis ponceau