Expression
Services
Cold osmotic shock - Concentration of inclusion bodies - Construction of recombinant Baculo virus - Generation of high titer stock (1 L) - Generation of high titer stock (100 mL) - Generation of high titer stock (500 mL) - MOI Assay of protein production
Cold osmotic shock 1 L
383,67 € (EUR) - Cold osmotic shock (1 L shake flask) to isolate periplasmatic proteins. - P2014.0000
Concentration of inclusion bodies 1 L
Price on request - Price depending on complete order. - P2013.0000
Construction of recombinant Baculo virus clone
5237,16 € (EUR) - 2 mL each of rec. virus supernatant as viral stock, plus 1 mL - P2017.0000
Generation of high titer stock (1 L) 1000 mL
3475,51 € (EUR) - Please select -details- for more information. - P2018.1000
Generation of high titer stock (100 mL) 100 mL
1227,69 € (EUR) - Please select -details- for more information. - P2018.0100
Generation of high titer stock (500 mL) 500 mL
2198,65 € (EUR) - Please select -details- for more information. - P2018.0500
Large Scale Protein Production set-up
Price on request - If you do need high/large amounts of protein we will develop stable cell lines. In 4 - 6 months gram quantities of protein can be produced by parameter controlled bioreactor runs. High producer cell l - P2026.0000
MOI Assay of protein production set-up
1194,92 € (EUR) - Infection of rec. Virus and control virus at one MOI of 10 flasks containing Sf9 cells. Harvesting of samples for the rec. and the control viruses at 5 time points. - P2019.0000
Production of proteins from rec. Baculo-viruses (1 L) 1 L
2560,54 € (EUR) - Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harv - P2020.1000
Production of proteins from rec. Baculo-viruses (20 L) 20 L
10856,69 € (EUR) - Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harv - P2020.1020
Production of proteins from rec. Baculo-viruses (5 L) 5 L
5121,08 € (EUR) - Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harv - P2020.5000
Production of proteins from rec. Baculo-viruses (500ml) 500 mL
1194,92 € (EUR) - Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harv - P2020.0500
Protein Expression (fermentor) 5 litres
3706,69 € (EUR) - Expression under optimised conditions in fermenter for production of higher amounts of protein. - P2011.0000
Protein Expression (flask) 1 L
699,07 € (EUR) - Expression under optimised conditions in Erlenmeyer flask. Harvest of cells and storage. Analysis per SDS-PAGE. - P2011.1000
Protein Expression Screening (E.coli). per condition
181,43 € (EUR) - Optimisation of expression conditions and screening of expression strains. Analysis by SDS-PAGE / Western. 3 mL test set-up. - P2011.0003
Transient Protein expression in mammalian cells set-up
Price on request - If you do need only small amounts of protein for activity tests or similar applications transient transfection is one of the most convenient methods to produce the required protein. In 10-14 days we c - P2025.0000
