Herstellung eines High Titer Stocks (1 L) 1000 mL
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Generation of high titer stock (1 L) 1000 mL
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Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of infection and harvesting of supernatant after 72 hours. Third round of infection in roller bottles or spinners with harvesting after 72 hours after infection. Viral titer is determined by plaque assay. Sterile filtration and mycoplasm test. |
Generation of high titer stock (1 L) 1000 mL - P2018.1000 |
Productnumber: P2018.1000
Productdetails:
Generation of high titer stock (1 L) 1000 mL
Matchcode: high titer stock baculo virus expression cloning Klonierung Genexpression
