Production of proteins from rec. Baculo-viruses (500ml) 500 mL

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Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harvesting of cells and/or cell culture supernatant. Protein production control by Immuno-Western-Blot. Pelleting of cells or concentration of supernatant.