DAF-2

1 mg

Fluorescent Dyes

5,6-Diaminofluorescein. DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.

Shows higher photostability than the classical fluorescein derivative DAF. Applications: DAF2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxideproducing neurons in brain slices, in mitochondria and in living plant cells.

Productnumber: S5439.0001

Technical Data: "CAS: [205391-01-1]; C20H14N2O5; MW = 362.34 g/mol. ?ex 505 nm; ?em 518 nm in 0.1 M Tris pH 8.0."

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DAF-2 1 mg  Chemicals