MGMT-CpG-Analysis

set-up

Genomics

MGMT (human) is located at chromosome 10 (10q26). Encoding the DNA repair protein O-Methylguanine-DNA Methyltransferase. Assay Data: The region of interest is within the promoter region of the MGMT gene, 27 CpG sites are observed in this ROI.

MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. Frequently loss of gene expression occurs via hypermethylation of CpG sites within the promoter region of the MGMT gene. Our Service: We offer a reliable and accurate assay service for analysing the methylation level of the promoter region of the human MGMT gene. Get each of the consecutive CpG sites analysed. Get detailled quantitative information about your samples. Analysing Method: 1) Genomic DNA is treated with bisulfite; 2) Amplification of the region of interest; 3) Methylation level of each CpG site is determined by inhouse Pyrosequencing®-Technology; 4) Quality Control of bisulfite rxn and sequence context are included.

Documentation:

  MGMT-CpG-Analysis set-up

Productnumber: G2003.0001

Productdetails:

  MGMT-CpG-Analysis set-up

Manual/Protocol:

  MGMT-CpG-Analysis set-up

Deutsche Produktseite:
MGMT-CpG-Analyse set-up  Services

Matchcode: cpg methylation snp pyrosequencing