HotStart Taq PCR Kit with dNTPs 1000 units
PCR
HotStart Taq PCR Kit with dNTPs 1000 units
|
HotStart Taq Polymerase with antibody, dNTPs (Mix), Buffer and MgCl2 provided in separate tubes. | |
|
The Genaxxon HotStart Taq Kit includes: 1000 units HotStart Taq Polymerase, 400µL of a 10mM dNTP-Mix, Buffer E with separate MgCl2 sufficient for up to 2000 x 25µL PCR reactions (0.5 units Taq per reaction). All items are in shipped in separate tubes. The Genaxxon HotStart Taq is a mixture of the normal Genaxxon Taq polymerase and a monoclonal antibody, which blocks polymerase activity prior to heating. The complex formed between the polymerase and the antibody prevents primer-dimer and other artifacts resulting from unspecific primer binding. The anitbody is inactivated while heating up above 75°C, requiring no prolonged first heating step prior to cycling. |
HotStart Taq PCR Kit with dNTPs 1000 units - M3009.100M |
Productnumber: M3009.100M
Productdetails:
HotStart Taq PCR Kit with dNTPs 1000 units
Manual/Protocol:
HotStart Taq PCR Kit with dNTPs 1000 units
Application: HotStart DNA polymerase for accurate PCR
Source: recombinant
Unit-definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Technical Data: 5 U/µL
Shipping/Storing-Information: shipped on blue-ice, store at -20°C
Matchcode: PCR Taq taq polymerase thermostable thermophilus aquaticus high processivity mixed with antibody for HOT START Hot Start HOT Start Hotstart amplification enzyme
