SuperHot Taq PCR Kit with dNTPs 1000 units

SuperHot Taq Polymerase, dNTPs (Mix), Buffer and MgCl2 provided in separate tubes.

The Genaxxon SuperHot Taq-Kit includes: 1000 units HotStart Taq Polymerase, 400µL of a 10mM dNTP-Mix, Buffer E with separate MgCl2 sufficient for up to 2000 x 25µL PCR reactions (0.5 units Taq per reaction). All items are in shipped in separate tubes. The Genaxxon bioscience SuperHot Taq polymerase is a modified form of thermostable DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and yield are increased compared to standard Taq-Polymerase. Additionally, difficult targets with high GC-content can be amplified. Sensitivity improves multiplex PCR, an applied PCR technique that amplifies several specific targets simultaneously. Applications that previously required two or more reactions can be performed in a single reaction tube. Hence, multiplexing represents a substantial saving o time and reagents.