AEBSF Hydrochloride

500 mg

4-(2-Aminoethyl)-benzolsulfonylfluoride - hydrochloride, Pefa-bloc

AEBSF is an irreversible inhibitor of Thrombin and other Serin proteases (Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin) by sulfonylation of a functional group in the active centre of the enzyme. AEBSF is much less toxic compared to PMSF and DFP and therefore recommended to substitute these reagents. AEBSF is highly soluble in water resulting in a solution of pH 6.5. At this pH AEBSF is most stable. We recommend to store stock solutions (20 mM or 100 mM in water or buffer) at -20°C for up to 2 months and prepare fresh working solutions. AEBSF is used at a working concentration of 0.1 - 0.2 mM. Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585; Nathan, D.F. & Linquist, S. (1995) Mol. Cell. Biol., 15, 3917-3925.

Productnumber: M6360.0500

Technical Data: Assay: min. 98.0% (HPLC); solubility (H2O): 10 g/L; MP = 181° - 185°C; MW = 239.69 g/mol; CAS: [30827-99-7]; C8H10FNO2SxHCl; most stable at pH around 6.5. Stable for 1 week at 4°C. Stock solution stable for 2 months at -20°C.

Shipping/Storing-Information: shipped at RT, store at +2°C to +8°C.

Deutsche Produktseite:
AEBSF Hydrochlorid 500 mg  Chemicals

Matchcode: AEBSF pefa bloc