T4 DNA-Ligase

10000 units

The enzyme catalyses the 5' - 3' synthesis of DNA from a primed single-stranded DNA template.

T4 DNA Ligase catalyzes the formation of a phosphodiester bonds between 5' phosphate and 3' hydroxyl termini in duplex DNA/RNA. This enzyme can join blunt end and cohesive end termini, repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. Cohesive End Ligation: For most cohesive end ligations, a 30 minute incubation at 20°C is sufficient. Incubations at 16°C for 4-16 hours are routinely used for the majority of applications. Ligation of blunt ends and single-base pair overhang fragments requires more enzyme to achieve the same extent of ligation as cohesive end DNA fragments. Ligation may be enhanced by addition of PEG, or by reducing the rATP concentration. ATP is an essential cofactor for the reaction.

Documentation:

  T4 DNA-Ligase 10000 units

Productnumber: M3027.1010

Productdetails:

  T4 DNA-Ligase 10000 units

Application: Cloning of restriction fragments, joining linkers and adapters to blunt-ended DNA, gene (gene fragments) synthesis.

Source: Isolated from E.coli cells infected with phage T4 amN82

Unit-definition: One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA in 30 minutes at 16°C at 5' termini concentration of 0.12 µM (300 µg/mL). One Cohesive End Ligation Unit equals 0.015 Weiss units. One Weiss unit equals 67 Cohesive End Ligation Units.

Technical Data: 50 - 100 CEL U/µL

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
T4 DNA-Ligase 10000 units  Cloning-Ligation

Matchcode: DNA synthesis cloning probe labelling blunt ends fill-in T4 enzyme