Basal Medium (Eagle) with HBSS with glutamine, with HEPES 500 mL

Basal Medium (Eagle) with Hank´s BSS, without L-Glutamine, with 25mM Hepes, with 0.35g/L NaHCO3, sterile filtered.

In the fifties of the last century it became clear that mammalian cells need not only the 10 essential amino acids, but also Cystine, Tyrosine and Glutamine. In addition to these three amino acids, BME includes also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa-cells. With its many variations it is used in many fields of cell culture and applications today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells.