Biotin-11-dUTP

1 mL

Modified Nucleotides Labelling of DNA by terminal deoxynucleotidyl transferase or DNA-polymerases.

1 mL of a 1 mM solution of Biotin-11-dUTP

Biotin-11-dUTP (Biotin-11-2'-deoxyuridin-5'-triphosphat - Tetralithiumsalt) is a commonly used component for non-radioactive labelling of DNA. Biotin-11-dUTP can be incorporated into DNA enzymatically by Nick-Translation, random-priming, 3'-end terminal labelling or by PCR. The figure '11' stands for the number of carbon atoms of the linker between dUTP and Biotin. The longer the 'linker-chain' the better the interaction between Biotin and Avidin. The shorter the linker the better the incorporation of dUTP into DNA. '11' has been shown to be a good compromise for most applications. The product is delivered as 1 mM solution in 10 mM Tris-HCl (pH7.5), 1 mM EDTA.

Documentation:

  Biotin-11-dUTP 1 mL

Productnumber: M3428.1000

Productdetails:

  Biotin-11-dUTP 1 mL

Application: Labelling of DNA by terminal deoxynucleotidyl transferase or DNA-polymerases.

Technical Data: 1 mM solution, 96% pure (IEC, TLC, NMR, UV).

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
Biotin-11-dUTP 1 mL  Molecular Biology

Matchcode: Biotin-11-dUTP