Modified Trypsin (Porcine)

5 x 20 µg

substance packed in 5 individually sealed vials

Highly purified modified Trypsin with extremely low autolysis and therefore greater control of protein fragmentation. Consistent Enzyme activity over long digestion periods. Characteristics: Trypsin is a serine endopeptidase which specifically cleaves peptide bonds on the carboxy side of Arginine, Lysine and s-aminoethyl cysteine residues. There is little or no cleavage at arginyl-proline or lysyl-proline bonds. Chemical Modification: This enzyme is subjected to extensive purification to remove contaminating proteases and tryptic autolysis by-products which could affect the specificity of the digestion process. Highly purified enzyme is modified chemically by a special process. As a result, the modified enzyme is more resistant to autolysis and has improved stability. The modified enzyme retains at least 90% of its activity after 6 hours' incubation at 30°C in reaction buffer and at least 70% of its activity after 24 hours of incubation under the same conditions with retention of specificity.

Productnumber: S5405.0520

Deutsche Produktseite:
Modifiziertes Trypsin (Schwein) 5 x 20 µg  Proteins & Enzymes