Calf Intestine Phosphatase (CIP)

1 g (20 U/mg)

The enzyme catalyses the removal of mononucleotides from 3'-hydroxyl termini of duplex DNA (preferred substrates are blunt or recessed 3'-termini).

Productnumber: M3035.0001

Source: calf intestine

Unit-definition: One unit is defined as the amount of enzyme which will hydrolyse 1 mikromole p-Nitrophenylphosphate in 1 minute at 37°C and pH 9.8 (Diethanolamine buffer). Unit-conversion: 1 Glycine unit equals about 3 DEA units, with 1 glycine unit is defined as the amount of enzyme hydrolysing 1 mikromole of p-Nitrophenylphosphate in 1 minute a 25°C and pH 9.6 (glycine buffer).

Technical Data: min. 20 U/mg protein; MW = ca. 80000 g/mol, CAS [9001-78-9], lyophilised.

Shipping/Storing-Information: shipped at RT, store at +2°C to +8°C

Deutsche Produktseite:
Calf Intestine Phosphatase (CIP) 1 g (20 U/mg)  Molecular Biology

Matchcode: removal of 5'-phosphate groups