Proteinase K Pulver 500 mg
Molekularbiologie
Proteinase K powder (>30U/mg) 500 mg
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An enzyme used for complete digestion of proteins in nucleic acid samples. | |
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Proteinase K, extracted from the fungus Tritirachium album, is a non-specific serine protease exhibiting a very broad cleavage range (cleaves the carboxylic ends of aromatic, hydrophobic and aliphatic amino acids) making it useful for general digestion of proteins in biological samples (Ebeling W. et al. (1974) Eur. J. Biochem., 47, 91). Proteinase K is mainly used in nucleic acid extraction protocols but may also be used in protein fingerprinting experiments, or for removal of nucleases. |
Proteinase K powder (>30U/mg) 500 mg - M3036.0500 |
Productnumber: M3036.0500
Productdetails:
Proteinase K powder (>30U/mg) 500 mg
Manualcertificate:
Proteinase K powder (>30U/mg) 500 mg
Application: Protein digest in DNA-probes
Source: Tritirachium album
Unit-definition: Activity: >30 U/mg. 1 unit is definded as the enzyme acitivity which liberates folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute at 37°C using haemoglobin as substrate.
Technical Data: Activity: min. 30 AnsonU/mg protein, DNAses and RNAses: not detectable; solubility: soluble in water, pH (1% H2O, 20°C): 6.2 - 6.8; appearance: white lyophilisate; MW = ca. 27,000 g/mol; CAS: [9036-06-0].
Shipping/Storing-Information: shipped at RT, store at +2°C to +8°C
Matchcode: Proteinase K
