Proteinase K powder

500 mg

An enzyme used for complete digestion of proteins in nucleic acid samples.

Proteinase K, extracted from the fungus Tritirachium album, is a non-specific serine protease exhibiting a very broad cleavage range (cleaves the carboxylic ends of aromatic, hydrophobic and aliphatic amino acids) making it useful for general digestion of proteins in biological samples (Ebeling W. et al. (1974) Eur. J. Biochem., 47, 91). Proteinase K is mainly used in nucleic acid extraction protocols but may also be used in protein fingerprinting experiments, or for removal of nucleases.

Productnumber: M3036.0500

Productdetails:

  Proteinase K powder 500 mg

Manualcertificate:

  Proteinase K powder 500 mg

Application: Protein digest in DNA-probes

Source: Tritirachium album

Unit-definition: One unit is the enzyme acitivity which liberates folin-positive amino acids and peptides corresponding to 1 µmole tyrosine in 1 minute at 37°C using haemoglobin as substrate.

Technical Data: "min. 30 mAnsonU/mg protein, DNAses and RNAses: not detectable; solubility: soluble in water, pH (1% H2O, 20°C): 6.2 - 6.8; appearance: white lyophilisate; MW = ca. 27000 g/mol; CAS: [9036-06-0]."

Shipping/Storing-Information: shipped at RT, store at +2°C to +8°C

Deutsche Produktseite:
Proteinase K Pulver 500 mg  Molecular Biology

Matchcode: proteinase endopeptidase