Proteinase K solution (20mg/mL)

5 x 1 mL

An enzyme used for complete digestion of proteins in nucleic acid samples.

Proteinase K, extracted from the fungus Tritirachium album, is a non-specific serine protease exhibiting a very broad cleavage range (cleaves the carboxylic ends of aromatic, hydrophobic and aliphatic amino acids) making it useful for general digestion of proteins in biological samples (Ebeling W. et al. (1974) Eur. J. Biochem., 47, 91). Proteinase K is mainly used in nucleic acid extraction protocols but may also be used in protein fingerprinting experiments, or for removal of nucleases.

Productnumber: M3037.0005

Productdetails:

  Proteinase K solution (20mg/mL) 5 x 1 mL

Manualcertificate:

  Proteinase K solution (20mg/mL) 5 x 1 mL

Application: Protein digest in DNA-probes

Source: Tritirachium album

Unit-definition: Concentration: 20mg/mL; Activity: >30 U/mg (>600U/mL). 1 unit is definded as the enzyme acitivity which liberates folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute at 37°C using haemoglobin as substrate.

Technical Data: Activity: min. 600 mAnsonU/mL; 20mg/mL proteinase K, CaCl2 x 2H2O: 1mM; Tris-HCl (pH 7.5): 10 mM; glycerin: 30%.

Shipping/Storing-Information: shipped and stored at RT

Deutsche Produktseite:
Proteinase K Lösung (20 mg/mL) 5 x 1 mL  Molecular Biology

Matchcode: Proteinase K