Ribonuclease A (DNAse free)

50 mg

RNAse A from bovine pancreas, salt free, lyophilised.

Ribonuclease A is a endo-ribonuclease specifically cleaving single stranded RNA at the 3' side of pyrimidine bases (cytosine and uracil). RNAse A ist used to prepare RNA-free DNA, to digest non-hybridisised regions of RNA-DNA hybrids and as a molecular weight marker. The pH optimum for RNAse A is between 7.0-7.5. RNAse A is inhibited by Diethylpyrocarbonate (DEPC), guanidinium salt (4 M GuaSCN), ß-Mercaptoethanol, heavy metals, vanadyl-ribonucleoside complexes, RNAse-inhibitor from human placenta and by competitive DNA). RNAse A cleaves single and douple stranded RNA and RNA in RNA:DNA hybrids at low salt conditions (100 mM NaCl). At high salt conditions (> 300 mM NaCl), RNAse A cleaves douple stranded RNA only. The enzyme can only be removed by Proteinase K digest and subsequent phenol extraktion.

Documentation:

  Ribonuclease A (DNAse free) 50 mg

Productnumber: S5218.0050

Application: "RNase protection assays; Remove unspecifically bound RNA; Analysis of RNA sequences; Hydrolyze RNA contained in protein samples; Purification of DNA; Pancreatic RNase A specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2'-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples."

Source: bovine pancreas

Unit-definition: "1 unit corresponds to the amount of enzyme which hydrolyzes RNA at a rate constant k = 1 at 25°C and pH 5.0 (Kunitz-units); M. Kunitz, J. Biol. Chem. 164, 563 (1946)."

Technical Data: "MW = ca. 13700 Da; no proteases detected; no DNAse activity detected; 90 KU/mg (Kunitz), E.C. 3.1.27.5, CAS [9001-99-4]."

Shipping/Storing-Information: shipped at RT, store at -20°C

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Ribonuclease A (DNAse frei) 50 mg  Molecular Biology

Matchcode: ribonuclease