Tth Inorganic Pyrophosphatase Thermostable

250 units

The enzyme catalyses the conversion of inorganic pyrophosphate to orthophosphate in a reaction where pyrophosphate is accumulated.

Native, thermostable Tth Inorganic Pyrophosphatase is a hydrolase purified from Thermus thermophilus. Tth Inorganic Pyrophosphatase catalyses the conversion of inorganic pyrophosphate to orthophosphate in a reaction where pyrophosphate is accumulated. These include DNA synthesis and amplification where Tth Inorganic Phosphatase provides enhanced polymerisation, by removing inhibiting amounts of pyrophosphates in the reaction.

Productnumber: M3013.0250

Productdetails:

  Tth Inorganic Pyrophosphatase Thermostable 250 units

Manual/Protocol:

  Tth Inorganic Pyrophosphatase Thermostable 250 units

Application: Enzyme for elimination of pyrophosphate for optimised PCR

Source: Thermus thermophilus

Unit-definition: One unit is defined as the amount of enzyme which is required to convert 1 µmole of pyrophosphate into 2 µmoles of orthophosphate in one minute at 75°C. Buffer conditions: 1 mM K4P4O7, 2 mM MgCl2, 50 mM Tris-HCl pH 9.0 (75°C).

Technical Data: 5 U/µL

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

Deutsche Produktseite:
Tth anorganische Pyrophosphatase 250 units  PCR-related

Matchcode: pyrophosphate sequencing PCR elimination pyrophosphatase