C-terminal Fluorescein modification of peptide set-up

C-terminal Fluorescein modification (or modification with other dye) of synthesised peptide. Synthesis range starting at 2mg with a purity of at least 70%. Please request price information for your peptide. For being able to make an appropriate quotation we do need the peptide sequence, the amount of peptide and the modification.

Biotin and FITC: For C-terminal labeling of biotin, a Lys residue is added to the C-terminus of the peptide. Biotin is then attached to the lysine side chain via amide bond. The positive charge of the lysine is then removed. Fluorescein isothiocyanate (FITC) is an activated precursor used for fluorescein labeling. For efficient N-terminal labeling, a seven-atom aminohexanoyl spacer (NH2-CH2-CH2-CH2-CH2-CH2-COOH) is inserted between the fluorophore (fluoroscein) and the N-terminus of the peptide.