SuperHot Taq-Polymerase (Special Offer)

1000 units

Modified Taq DNA Polymerase for HotStart and Multiplex PCR.

The Genaxxon BioScience SuperHot Taq polymerase is a modified form of thermostable DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and yield are increased compared to standard Taq-Polymerase. Additionally, difficult targets with high GC-content can be amplified. Sensitivity improves multiplex PCR, an applied PCR technique that amplifies several specific targets simultaneously. Applications that previously required two or more reactions can be performed in a single reaction tube. Hence, multiplexing represents a substantial saving o time and reagents.

Documentation:

  SuperHot Taq-Polymerase (Special Offer) 1000 units

Productnumber: M3307.1000

Productdetails:

  SuperHot Taq-Polymerase (Special Offer) 1000 units

Manual/Protocol:

  SuperHot Taq-Polymerase (Special Offer) 1000 units

Application: For multiplex PCR. For Real-time PCR, for PCR of low amount of DNA (very low copy number).

Source: recombinant

Unit-definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Technical Data: 5 U/µL; Enyzme is completely inactive prior to heating at 95°C for at least 10 min.!

Shipping/Storing-Information: shipped on blue-ice, store at -20°C

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SuperHot Taq-Polymerase für qPCR (Angebot) 1000 units  Special Offers

Matchcode: PCR Taq taq polymerase thermostable thermophilus aquaticus high processivity chemically modified HOT START Hot Start HOT Start Hotstart amplification enzyme