BCIP, molecular biology grade,

1 g

Substrates

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase, used in combination with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development. Applications: When alkaline phosphatase conjµgates are used for detection, BCIP is recommended for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots. It is recommended to prepare a 50 mg/mL stock solution in DMF. Protocol for Preparation of BCIP/NBT Substrate Solution: 1. Add the following components to 10 ml of the Alkaline Phosphatase (AP) buffer (100 mM Tris-HCl (pH 9.5), 100 mM NaCl and 10 mM MgCl2): 33 µL BCIP (50 mg/mL in DMF) plus 44 µL NBT (75 mg/mL in 70% DMF). NOTE: Avoid exposure to light - Prepare fresh developing solution and use within an hour - BCIP and NBT solutions in DMF do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.

Productnumber: M3196.0001

Technical Data: "MW = 433.6 g/mol; min. 99% (HPLC); pH (5% in water): 1.0-2.0; solubility (water, 20°C): 582 g/L; MP = 120°C; chloride: max. 0.001%, Pb: max. 0.005%, Mn: max. 0.00005%; free acid: max. 0.1%; residue on ignition: max. 0.005; CAS: [6578-06-9]."

Shipping/Storing-Information: shipped at RT, store at -20°C

Deutsche Produktseite:
BCIP für die Molekularbiologie 1 g  Biochemicals

Matchcode: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt C8H6NO4BrCIPxC7H9N BCIP CAS: 6578-06-9