TMB ready-to-use solution 1 L

TMB ready-to-use ELISA substrate.

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5M H2SO4, which turns the substrate yellow (450 nm). One bottle of EC Blue consists of a solution containing proprietary ready-mixed chromogen substrate reagents, pH3.8. The solution has a straw-yellow colour at room temperature and a light greenish tint when stored at 4°C. The colour returns to normal when warmed overnight to room temperature. Light sensitive! Don't freeze!