X-Gal, Molecular biology grade (min. 99.0%) 1 g

5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside

For identification of lac(f)-colonies. Histochemical substrate for b-galactosidase. X-Gal is well a well known histochemical substrate used to detect the b-galactosidase enyzme. X-gal is used to distinguish recombinant plasmids from parental vectors cloning experiments using vectors containing the lacZ gene. Upon hydrolysis, X-gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical and ELISA assays. Stock solution: 20mg/mL in DMF. Store aliquots at -20°C in the dark. For detection of transformants, use in final concentration of 40µg/mL.