X-Gal, Molecular biology grade (min. 99.0%)

1 g

5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside

For identification of lac(f)-colonies. Histochemical substrate for b-galactosidase. X-Gal is well a well known histochemical substrate used to detect the b-galactosidase enyzme. X-gal is used to distinguish recombinant plasmids from parental vectors cloning experiments using vectors containing the lacZ gene. Upon hydrolysis, X-gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical and ELISA assays. Stock solution: 20 mg/mL in DMF. Store aliquots at -20°C in the dark. For detection of transformants, use in final concentration of 40 µg/mL.

Productnumber: M3204.0001

Manualcertificate:

  X-Gal, Molecular biology grade (min. 99.0%) 1 g

Technical Data: "min. 99.0% (HPLC), MW = 408.6 g/mol, MP: 223°C; a20°C/D: 1%, 50% DMF: -62° +/- 2°; water (K.F.): max: 1%; CAS: [7240-90-6]."

Shipping/Storing-Information: "store at -20°C in the dark; keep away from light"

Deutsche Produktseite:
X-Gal, (min. 99,0%) 1 g  Biochemicals

Matchcode: 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside X-Gal chromogenic substrate b-galactosidase