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FAQs about SNP DNA Polymerase HiDi

Can I use HiDi DNA Polymerase with bisulfite-treated DNA samples?

Yes, you can. Our HiDi DNA Polymerase is for example performing very well in methylation specific PCR (MSP). Since a single mismatch is sufficient for specific MSP results, HiDi DNA Polymerase even enables reliable MSP analysis of single CpG sites.

Can I use HiDi 2x PCR Master Mix and HiDi DNA Polymerase in probe-based assays?

Yes, you can use it in probe assays, which don't require a nuclease activity (e.g., HybProbe or SimpleProbe). Please be aware that HiDi DNA Polymerase doesn't have a 5'-3'-nuclease activity, so you can't use HiDi 2x PCR Master Mix in hydrolysis-based probe assays (e.g., TaqMan®). For this assays, we recommend to use our HiDiTaq DNA Polymerase with 5'-3'-nuclease activity. HiDiTaq variant can therefore be used for hydrolysis probe-based real-time PCRs. 

What is the error-rate of HiDi DNA Polymerase?

HiDi's error-rate is comparable to the error-rate of wildtype Taq DNA Polymerase.

Can I use HiDi DNA Polymerase in real-time PCRs using a real-time dye such as SYBR Green?

Please be aware that HiDiTaq DNA Polymerase is not suitable for real-time PCRs using a real-time dye. HiDi DNA Polymerase works very well with real-time dyes.

Can I use Direct Oral Swab 2x PCR Master Mix in probe-based assays?

Yes, HiDiTaq DNA Polymerase has a 5'-3'-nuclease activity, so you can use Direct Oral Swab 2x PCR Master Mix in probe-based assays.

For which amplicon length should primers be designed?

We recommend designing primers with a short amplicon length (about 60-200 bp) for best results, but also longer amplicon lengths are possible.

Where can I read more about HiDi DNA Polymerase?

HiDi DNA polymerase is based on scientific results, which are published in the open access and peer-reviewed Journal PLOS ONE: 

> Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification for high discrimination.

Where does the name HiDi come from?

HiDi stands for High Discrimination.