CHAPS buffer grade 5 g - M6258.0005

Package Size: 5 g CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).


Technical Data: Purity: >99.0% (HPLC); MW = 614.89 g/mol; White solid crystals; CMC 4.2 - 6.3 mmol/L; Aggregation No.: 10; Al: max. 0.0005%; Ca: max. 0.0005%; Cu: max. 0.0005%; Fe: max. 0.0005%; Mg: max. 0.0005%; K: max. 0.005%; Pb < 0.005%; Na < 0.005%; Zn: max. 0.0005%; H2O: max. 3%; conductivity: max. 10 µS/cm (25°C, 1% solution); C32H58N2O7S; CAS: [75621-03-3].

Shipping / Storing Information: shipped at RT, store at +2°C to +8°C

Productnumber: M6258.0005

changedate: 23.8.2010

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