Methylen Blue (C.I. 52015) 25 g - M3359.0025

Package Size: 25 g Methylenblue is used to stain nucleic acids in polyacrylamide gels, but it can also be used to stain agarose gels. Staining procedure: Incubate gel for 1 hour in 0.4 M Na-acetate/0.4 M acetic acid / 0.2% methylenblue (pH4.7). Peacook A.C., Dingman C.W. (1967) Biochemistry 6, 1818-1827.


Application: Used to stain RNA in polyacrylamide gels at pH below 6.0.

Technical Data: min. 85% (photometric); lambda(max): 663-667nm; E 1%/1cm, lambda(max): max. 15%; solubility (20°C): 50 g/L in water; MP = 180°C; C16H18ClN3S; MW = 319.86 g/mol; CAS: [61-73-4].

Shipping / Storing Information: shipped and stored at RT

Productnumber: M3359.0025

changedate: 30.4.2010

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