Ponceau S Solution 100 mL - M3350.0100

Package Size: 100 mL Ponceau S is used for reversible staining proteins blotted on nitrocellulose or PVDF membrans and while running a TAU electrophoresis. The reversibility of the staining process is ideally suited for subsequent immunoblots (western blot) or other colour reactions as Ponceau S can be completely removed. Ponceau S is not stable for long time, thus proteins and protein markers should be marked with a pencil. For staining the membrane (nitrocellulose or PVDF) is incubated in a 0.1% - 0.5% Ponceau S solution in 5% acetic acid (alternatively in 3% trichloracetic acid) for 5 - 10 minutes. Destaining is done by incubating the stained membrane in 5% acetic acid for 15 minutes. Our Ponceau S solution is offered as 0.1% Ponceau S (w/v) in 5% acetic acid (v/v).

Ponceau S Solution Productdetails (pdf)

Application: for reversible staining of blotted membranes

Technical Data: density (d 20°/4°): 1.01; n 20°/D: 1.338; composition: 50.00g/L acetic acid; 1.0g/L ponceau S.

Shipping / Storing Information: shipped and stored at RT

Productnumber: M3350.0100

changedate: 7.1.2011

Print product data sheet