Ni-NTA-Agarose 5 mL - S5377.0005

Package Size: 5 mL Genaxxon Ni-NTA Agarose provides a simple and very effective means of purifying recombinant proteins fused with a 6-fold histidine tag (His-tag). The His-tag does not usually affect the bioactivity of the protein in question, and the purification procedure is simple. The agarose consists of the tetradentate chelating agent, nitrilotriacetic acid (NTA), covalently coupled to 6 % cross-linked agarose beads, and is loaded with Ni2+ ions. Immobilized Nickel selectively binds exposed histidine residues and allows recombinant His-tagged proteins to be purified directly from crude bacterial lysates under either native or denaturing conditions. Resin allows batch or column purifications. Performance equals or exceeds any popular Ni-NTA-Agarose from other suppliers.

Ni-NTA-Agarose Manual / Protocol (pdf)

Technical Data: 6% cross-linked Agarose, grain size: 50 – 170µm (spherical); max. pressure: 0.18 bar (2.6 psi); binding capacity: >50mg of 6xhistidine tagged fusion protein per 1mL agarose.

Shipping / Storing Information: shipped at RT, store at +2°C to +8°C

Productnumber: S5377.0005

changedate: 30.12.2011

Print product data sheet