Highflow Ni-IDA Agarose 500 mL - S5354.0500

Package Size: 500 mL Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high yields result from purification of these fusion proteins from the lysates. However, if purification takes place from lysates containing foreign protein chains with repeated histidine elements, selectivity with respect to 6×histidine-tagged fusion protein decreases as non-tagged histidine-containing proteins increasingly also bind to the Ni-IDA agarose. In this case the use of Genaxxon Co-IDA Agarose is recommend (cat#: S5370), which features far higher selectivity with respect to 6×histidine-tagged fusion protein. Genaxxon Ni-IDA Agarose has high chemically and pH stability and enables purification directly from raw lysates. Genaxxon Ni-IDA Agarose can be regenerated several times with nickel sulfate solution for re-use. Highflow Ni-IDA-Agarose (High Pressure) - a highly efficient and favorable way for the separation of 6-His tagged fusion proteins with high pressure procedures. Highlights: ideal for downstream procedures - highly applicable for high amounts of 6-His tagged proteins - applicable for peristaltic pump systems (FPLC(TM), Äkta(TM), MPLC) - purification direct from raw lysate under native or denaturing conditions - easy to up-scale - autoclavable - regenerative - cheap. The Genaxxon Highflow Ni-IDA Agarose consists of 6% cross-linked Agarose, covalently bound to Iminodiacetic acid loaded with Ni2+-ions. Binding capacity: ca. 20µmol Me2+ per mL Agarose. Application: Affinity chromatography of 6-His tagged fusion proteins in downstream procedures. Ni-IDA Agarose is supplied as buffered suspension in 20% Ethanol.


Technical Data: 6% highly cross-linked Agarose, grain size: 50 – 150µm (spherical); max. flow rate: >600cm/h at 15cm bed height and 100 kPa; max. pressure: 300 kPa (at 15cm bed height); ca. 20 µmol Me2+/mL resin; chemical stability: stable in all commonly used reagents; autoclavable at 121°C for 30 min..

Shipping / Storing Information: shipped at RT, store at +2°C to +8°C

Productnumber: S5354.0500

changedate: 11.6.2011

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