DF Taq Polymerase (DNA-free) 2 x 250 units - M3185.0500

Package Size: 2 x 250 units The Genaxxon bioscience DF-Taq DNA Polymerase is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to 10 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15 mM MgCl2. The special purification procedure guarantees Taq-Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene.

DF Taq Polymerase (DNA-free) Product Details Document (pdf)
DF Taq Polymerase (DNA-free) Productdetails (pdf)
DF Taq Polymerase (DNA-free) Manual / Protocol (pdf)
DF Taq Polymerase (DNA-free) Manual / Certificate (pdf)

Application: DNA polymerase to be used for PCR of E.coli DNA or for any 16S typisation applications.

Source: Thermus aquaticus strain

Technical Data: 5 U/µL

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3185.0500

changedate: 12.5.2011

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