SuperHot Taq PCR Kit with dNTPs 250 units - M3306.025M

Package Size: 250 units The Genaxxon SuperHot Taq-Kit includes: 250 units HotStart Taq Polymerase, 100µL of a 10mM dNTP-Mix, Buffer E with separate MgCl2 sufficient for up to 500 x 25µL PCR reactions (0.5 units Taq per reaction). All items are in shipped in separate tubes. The Genaxxon bioscience SuperHot Taq polymerase is a modified form of thermostable DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and yield are increased compared to standard Taq-Polymerase. Additionally, difficult targets with high GC-content can be amplified. Sensitivity improves multiplex PCR, an applied PCR technique that amplifies several specific targets simultaneously. Applications that previously required two or more reactions can be performed in a single reaction tube. Hence, multiplexing represents a substantial saving o time and reagents.

SuperHot Taq PCR Kit with dNTPs Product Details Document (pdf)
SuperHot Taq PCR Kit with dNTPs Manual / Protocol (pdf)

Application: For multiplex PCR. For Real-time PCR, for PCR of low amount of DNA (very low copy number).

Source: recombinant

Technical Data: 5 U/µL; Enyzme is completely inactive prior to heating at 95°C for at least 10 min.!

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3306.025M

changedate: 10.1.2011

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