Pwo Polymerase 2 x 250 units - M3002.0500

Package Size: 2 x 250 units Genaxxon bioscience Pwo DNA Polymerase is a highly processive 5' - 3' DNA Polymerase with additional 3' - 5' exonuclease activity (proof-reading). The Genaxxon bioscience Pwo DNA Polymerase is the recombinant form, originally isolated form the themophilic archaebacterium Pyrococcus woesei. It has been cloned and expressed in E.coli. The enzyme has no detectable 5' - 3' exonuclease activity. It exhibits increased thermal stability as well as a tenfold increase in fidelity of DNA synthesis compared to Taq DNA Polymerase. Pwo generates blunt-ended PCR products.

Pwo Polymerase Product Details Document (pdf)
Pwo Polymerase Productdetails (pdf)
Pwo Polymerase Manual / Protocol (pdf)
Pwo Polymerase Manual / Certificate (pdf)

Application: Proof-reading DNA polymerase for high fidelity PCR.

Source: recombinant

Technical Data: 2.5 U/µL

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3002.0500

changedate: 12.5.2011

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