ReproHot Polymerase 250 units - M3012.0250

Package Size: 250 units ReproHot is a mixture of Taq polymerase plus a proof-reading polymerase and a monoclonal antibody, which blocks polymerase activity prior to heating. The complex formed between the polymerase and the antibody prevents primer-dimer and other artifacts resulting from unspecific primer binding. The antibody is inactivated while heating up above 75°C, requiring no prolonged first heating step prior to cycling. The combined hotstart and proof-reading feature leads to highly specific and correct amplicons. ReproHot can be used as alternative to KOD-Polymerase.

ReproHot Polymerase Product Details Document (pdf)
ReproHot Polymerase Productdetails (pdf)
ReproHot Polymerase Manual / Protocol (pdf)
ReproHot Polymerase Manual / Certificate (pdf)

Application: HotStart DNA polymerase for accurate PCR

Source: recombinant

Technical Data: 5 U/µL

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3012.0250

changedate: 12.5.2011

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