Tth Polymerase 500 units - M3005.0500

Package Size: 500 units Genaxxon bioscience Tth DNA Polymerase is the recombinant form of the enzyme obtained from the thermophilic eubacterium Thermus termophilus HB-8. It is a highly processive 5' - 3' DNA Polymerase lacking 3' - 5' exonuclease activity. Tth DNA Polymerase catalyses the polymersisation of nucleotides into duplex DNA in the 5' - 3' direction in the presence of MgCl2 together with a very efficient intrinsic reverse transcriptase (RT) activity in the presence of Mn2+ ions. This RT activity is not associated with RNase H activity. The ability of Tth DNA Polymerase to catalyse the polymerisation of DNA, using a RNA template at high temperature, minimises the problems encountered with strong secondary structures in RNA, since they are unstable at higher temperatures. Tth DNA Polymerase is resistant to prolonged incubation at 95°C.

Tth Polymerase Product Details Document (pdf)
Tth Polymerase Productdetails (pdf)
Tth Polymerase Manual / Protocol (pdf)

Source: recombinant

Technical Data: 5 U/µL

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3005.0500

changedate: 12.5.2011

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