Pfunds proof reading polymerase 250 units - M3004.0250

Package Size: 250 units The Genaxxon bioscience Pfunds-Polymerase is a thermostable enzyme possessing 5'-3' DNA polymerase and 3'-5' proof reading exonuclease activities. It is isolated from the hyperthermophilic marine archae Pyrococcus furiosis (Pfu). The enzyme provides extremely high fidelity. Whereas the enzyme is not able to amplify long fragments as efficiently as Taq-Polymerase because of its very high exonuclease activity, a mixture of Taq-Polymerase and Pfunds-Polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220).

Pfunds proof reading polymerase Product Details Document (pdf)
Pfunds proof reading polymerase Productdetails (pdf)
Pfunds proof reading polymerase Manual / Protocol (pdf)
Pfunds proof reading polymerase Manual / Certificate (pdf)

Application: Proof-reading DNA polymerase for high fidelity PCR.

Source: Pyrococcus furiosis

Technical Data: 2.5 U/µL

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3004.0250

changedate: 12.5.2011

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